J Pharmacol Exp Ther. However, administration of Z. zerumbet extract appeared to reduce oxidative stress induced by ethanol as evidenced by increased level of GSH in the brain.  that demonstrated ethanol administration at the dose of 1.8 g/kg via intraperitoneal for 14 days caused neurodegeneration in the cerebral cortex. It is locally known as ‘Lempoyang’ and traditionally used to treat fever, constipation and to relieve pain. Halliwel B. Reactive species and antioxidants. It likes shade, and can be grown from bright shade to dark shade. Zingiber zerumbet(L) Smith, commonly known as the pinecone or shampoo ginger, is a perennial, tuberous root herb plant that has gained much interest from scientists all over the world because of its high medicinal values. Zingiber zerumbetis widely used as therapeutic agent in traditional medicine and is reported to exert antioxidant activities. CAT activity was measured by Aebi method  and its principle was based on the ability of CAT enzymes to catalyze the reduction of H2O2 to O2. THM performed the in vivo study. The extract of Z. zerumbet was given once daily by oral gavage, 30 min prior to ethanol exposure via intraperitoneal route for 14 consecutive days. By using this website, you agree to our Furthermore, the antioxidant activity of Z. zerumbet and its constituents were also found to be able to eliminate free radicals and its antioxidant activities were increased in a dose-dependent manner . 2002;26(4):547–57. Another study on oral administration of ethyl acetate extract of Z. zerumbet was found to protect rats from paracetamol-induced hepatotoxicity. 2012;7(3):81–8. Obernier JA, Bouldin TW, Crews FT. Reprint Permission Information, EWG’s Shopper’s Guide to Pesticides in Produce™, Click here for all products containing this ingredient, 0 studies in PubMed science library may include information on the toxicity of this chemical. For the brain samples, only 400 mg/kg Z. zerumbet extract showed significant increased (p < 0.05) in the activities of these three enzymes as compared to the ethanol-induced group. 2006; https://doi.org/10.1186/1472-6882-6-12. 2016;26(3):385–91. This characteristic is in agreement with Phachonpai et al. Note the number and shape of neuron cells. Ibrahim MY, Abdul AB, Ibrahim TAT, Wahab SIA, Elhassan MM, Mohan S. Attenuation of cisplatin-induced nephrotoxicity in rats using zerumbone. Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations. Asian J Pharm Clin Res. (N = 6). statement and Based on this finding, Z. zerumbet ethyl-acetate extract is proven to possess protective effects against ethanol-induced brain damage in Wistar rats by decreasing lipid and protein oxidation as well as increasing antioxidant activities. Protein carbonyl level was determined according to a method as described by Levine  based on the reaction of carbonyl compounds with 2, 4-dinitrophenyl hydrazine (DNPH). http://creativecommons.org/licenses/by/4.0/, http://creativecommons.org/publicdomain/zero/1.0/, https://doi.org/10.1186/s12906-018-2161-5. That is why both cerebral cortex and cerebellum are the main target in this study. Baker KG, Harding AJ, Halliday GM, Kril JJ, Harper CG. 1998;105(2):159–66. Cancer Cell Int. Correspondence to Alcohol causes oxidative damage by producing superoxide, hydroxyl and hydroethyl radicals which may contribute to lipid peroxidation, protein carbonyl formation and DNA damage . A significant increased (p < 0.05) level of MDA was observed in brain samples of ethanol-induced group as compared to the normal group. The ability of Z. zerumbet to protect brain tissues from lipid peroxidation and protein oxidation is in agreement with Hamid et al. The rats were then sacrificed. Butterfield DA, Castegna A, Drake J, Scapagnini G, Calabrese V. Vitamin E and neurodegenerative disorders associated with oxidative stress. Article Acute ethanol administration oxidatively damages and depletes mitochondrial DNA in mouse liver, brain, heart, and skeletal muscles: protective effects of antioxidants. 2001;298(2):737–43. Wudpecker J Food Technol. 2006;141(2):312–22. ZZRext contains high phenolic and flavonoid contents. Anti-Microbial - In this study they investigated antimicrobial effects of ethanolic extract of Zingiber zerumbet (Z. zerumbet) (L.) Smith and its chloroform and petroleum ether soluble fractions against pathogenic bacteria and fungi. This This study was conducted to evaluate the effects of Z. zerumbet extract in gout-induced rats. Thus, the aim of this study is to investigate the protective effects of the ethyl-acetate extract of Z. zerumbet rhizomes against ethanol-induced brain damage in male Wistar rats. Blood was collected by cardiac puncture in plain tubes and centrifuged at 3000 rpm for 10 min to obtain serum. Neuronal loss in functional zones of the cerebellum of chronic alcoholics with and without Wernicke’s encephalopathy. This was shown by increased in both antioxidant SOD and GSH level and reduction of both homogenate and plasma MDA, plasma protein carbonyl, and renal advanced oxidation protein product (AOPP) than those in the paracetamol induced group . Abdul Hamid Z, Budin SB, Wen Jie N, Hamid A, Husain K, Mohamed J. Nephroprotective effects of Zingiber zerumbet Smith ethyl acetate extract against paracetamol-induced nephrotoxicity and oxidative stress in rats. The authors declare that they have no competing interests. 2011; https://doi.org/10.1155/2011/543216. Before a company can use EWG VERIFIED™ on such products, the company must show that it fully discloses the products' ingredients on their labels or packaging, they do not contain EWG ingredients of concern, and are made with good manufacturing practices, among other criteria. Lipnik-Štangelj M. Ethanol toxicity in the brain: alteration of Astroglial cell function. Besides that, protein is also one of the targets of ROS and its oxidation will cause protein to lose its functions. Review of the experimental data and implications to humans. Beyer WF, Fridovich I. Assaying for superoxide dismutase activity: some large consequences of minor changes in conditions. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. The procedure was followed by two steps of washing using ethanol/ethyl-acetate that would remove lipids from the protein pellet. 1959;82(1):70–7. This suggest that Z. zerumbet has the potential as a neuroprotective agent. Zingiber aromaticum Valeton. 2003;25(1):1–9. 2009;30(1–2):1–12. After a few weeks, the first green cone turns red and then flowers appear in the cone. (EtOH: ethanol; 200 + EtOH: 200 mg/kg extract + ethanol; 400 + EtOH: 400 mg/kg extract + ethanol). Zingiber sylvestre Garsault. This suggest that the decreased in the CAT activity may be due to the loss of nicotinamide adenine dinucleotide phosphate (NADPH), anion (O2•¯) production and lipid peroxidation . Treatment with Z. zerumbet extract at 200 mg/kg and 400 mg/kg significantly increased (p < 0.05) the SOD, CAT and GPx enzyme activities in serum samples. Oxidative stress induced by short-term alcohol-intake  ultimately lead to neuronal cell death . L. and Laurus nobilis. Malondialdehyde (MDA) level is an index to determine lipid peroxidation process (Ledwozyw et al. Free Radic Biol Med. 2008;23(4):341–4. Brain tissues samples were fixed in 10% formalin and dehydrated in an ascending series of alcohol concentration (50% to 100%) and then embedded in paraffin blocks. Affandi MMR, Teh LK, Salleh MZ, Zakaria ZA. Serum samples were stored at − 20 °C for subsequent biochemical analysis. Zingiber zerumbet (L.) Roscoe ex Sm. Although it is metabolized mainly in the liver, it also causes toxic effects to the brain . A qualitative study found that there was a loss of Purkinje cells in the vermis with a reduction by 43% on average . 1b). Hamid A, Budin SB, Mohamed RAP, Abd Manaf N, Yuhana NY, Husain K, Abdul Hamid Z, Mohamed J. About the Experts. STZ-diabetic rats were treated orally with ZZRext (200, 300 mg/kg per â¦ 2011;67:209–25. Alcohol is a commonly abused substance worldwide that generates reactive oxygen species (ROS) whether it is consumed acutely or chronically . Anal Biochem. (FSK/BIOMED/2012/ASMAH/21-NOV/475-NOV-2012-JUNE-2013). Nicotinamide adenine dinucleotide phosphate. Developmental & Reproductive Toxicity . Assessment report on Zingiber officinale Roscoe, rhizoma . Article Learn more | Legal Disclaimer, Beyond providing Skin Deep® as an educational tool for consumers, EWG offers its EWG VERIFIED™ mark as a quick and easily identifiable way of conveying personal care products that meet EWG's strict health criteria. Springer Nature. Marella S, Reddy KS. In addition, an increase in the antioxidant activity can be described as an adaptive response to excessive ROS . Zingiber zerumbet rhizomes were supplied from herbs farm (Kizaherbs Sdn Bhd) in Temerloh, Pahang, Malaysia. (N = 6). BMC Complement Altern Med 18, 101 (2018). Zingiber zerumbet (L) Smith well known as lempuyang, is a wild ginger belonging to the Zingiberaceae family, and has been used as an ingredient in some traditional medicines. Brain is rich in polyunsaturated fatty acids and iron. The current study showed that ethanol decreased CAT activity in the brain after 14 days of alcohol exposure. (EtOH: ethanol; 200 + EtOH: 200 mg/kg extract + ethanol; 400 + EtOH: 400 mg/kg extract + ethanol). Maralla S, Reddy KS. This useful and medicinal Polynesian â¦  and Abdul Hamid et al. J Neurosci. A total of 24 male albino Wistar rats were obtained from Laboratory Animal Resources Unit, Universiti Kebangsaan Malaysia (UKM). Values are expressed as mean ± SEM. The prepared slides were observed under light microscope (Olympus BX41 Japan). Search by keywords . Zingiber zerumbet has been traditionally used as an anti-inflammation and antioxidant agent. Thomas JD, Goodlett CR, West JR. Alcohol-induced Purkinje cell loss depends on developmental timing of alcohol exposure and correlates with motor performance. All datasets used and/or analysed during the current study available from the corresponding author on reasonable request. Rains JL, Jain SK. Figure 3a showed that no histological changes were observed in the normal, 200 mg/kg + ethanol, and 400 mg/kg + ethanol groups as evidenced by normal Purkinje cells in the cerebellum (Fig. In: Tortora GJ, Derrickson B, editors. However, protein carbonyl levels were found to be significantly lower (p < 0.05) in both Z. zerumbet treated group (200 mg/kg and 400 mg/kg) as compared to the ethanol-induced group (Fig. PubMed online scientific bibliography data. Increased in the lipid peroxidation is represented by an increase in the MDA concentration, which is a good biomarker for oxidative stress in the brain . The relationship between plasma triglycerides, cholesterol, total lipids and lipid peroxidation products during human atherosclerosis. 2008;108(3):227–38. Representative images for In Vitromigration assay of human dermal fibroblast adult (HDF-a) cells treated with ethyl acetate extract of Zingiber zerumbet at 24, 48, 72 and 96 h (×40). Wattanathorn J, Phunchago N, Muchimapura S, Thukhum-Mee W, Chaisiwamongkol K, Kaewrueng W, Wongareonwanakij S. Mulberry fruit mitigates alcohol neurotoxicity and memory impairment induced by chronic alcohol intake. Aust J Basic Appl Sci. Lipids are biomolecules that often become the target of oxidative stress and its oxidation will produce many secondary products such as MDA. Some probably aren't as safe as you'd hope. Pharmacology. Otse pealehele Estonian. Zingiber officinale extract was a potential brain tonic to enhance cognitive function for ethanol-related cognitive deficits . Supplementation with Z. zerumbet extracts at 200 mg/kg and 400 mg/kg significantly decreased (p < 0.05) MDA levels compared to the ethanol-induced group (Fig. Phachonpai W, Wattanathorn J, Wannanon P, Thipkaew C, Sripanidkulchai B, Muchimapura S. Coscinium Fenestratum protects against ethanol-induced neurodegeneration in adult in rat brain. Studies on antioxidant activity of Zingiber zerumbet spent and its constituents through in vitro models. Legal Disclaimer | 2013;1(3):48–55. Z. zerumbet extract protects against ethanol-induced brain damage via its antioxidant properties. Data were expressed as mean ± standard error of mean (SEM). 2g). As the brain is rich in lipid content, it has high oxygen demand and lacks antioxidant activities thus very susceptible to oxidative stress as the brain easily undergo redox-reaction leading to radicals formation . (P- Purkinje cell; ML-Molecular layer; GL-Granular layer). â¦ Data normality and homogeneity were determined using Shapiro-Wilk and Levine test. 2011;17:854–63. Ledwozyw A, Michalak J, Stepień A, Kadziołka A. BMC Complementary and Alternative Medicine Mansouri A, Demeilliers C, Amsellem S, Pessayre D, Fromenty B. Evid Based Complement Alternat Med. Zingiber zerumbet (Smith) belongs to the family Zingiberaceae and is locally known as “lempoyang” . New York: Wiley; 2005. p. 931. Thus, Z. zerumbet extract is proven not only to increase the antioxidant enzyme activities in the liver and kidney but also in the brain. Zingiber Zerumbeti ekstrakti tootjad Bolise'ist. 1999;91(2):429–38. 2012. The plant extract was found to exhibit protective capability and cognitive â¦ Ang mga gumagawa ng Zingiber Zerumbet Extract mula sa Bolise. Cohen-Kerem R, Koren G. Antioxidants and fetal protection against ethanol teratogenicity I. She is known worldwide as The Cosmetics Cop and creator of Paulaâs Choice Skincare. Increased SOD, CAT and GPx activities in this study may relate to the fact that the high antioxidant activities in Z.zerumbet extract induced endogenous antioxidant thus reduced the free radical activity . In addition, Hamid et al. It was also shown that Z. officinale at the dose of 200 mg/kg body weight could protect the brain against ischemic brain damage and reduced cognitive deficits in a rat model of focal cerebral ischemia induced by the occlusion of the right middle cerebral artery. Brain Res Dev Brain Res. Free Radic Biol Med. Brain samples were washed with normal saline and homogenized in cold 1.15% potassium chloride (KCI) (3 ml/g) using Ultra Turrax T25 homogenizer (IKA, Germany). Indian J Clin Biochem. High lipid content and aerobic metabolism activities in nervous tissues make them more susceptible to oxidative damage . PubMed Google Scholar. 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